Unravelling the beehive air volatiles profile as analysed via solid-phase microextraction (SPME) and chemometrics

Abstract Objective Beehive air therapy is recognized as a potential remedy for treating asthma, bronchitis, lung fibrosis, and respiratory tract infections. Developed countries in which beehive currently authorized include Germany, Hungary, Slovenia, Austria. However, scientific proof of its efficacy lacking warrants further chemical biological analyses concept. In this study, volatile profile was determined the first time along with individual components (bees, venom, honey, beeswax). Methods Volatile compounds were collected from using solid phase micro-extraction (SPME) coupled to gas chromatography-mass spectrometry (GC–MS). Antimicrobial assay released 4 products performed against Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, multi drug-resistant aureus (MRSA) vitro agar-well diffusion microtiter plate assays. Results conclusions: A total 56 identified air, bee insect wax including 6 fatty acids, alcohols, 10 aldehydes, 5 esters, 1 ether, 9 hydrocarbons, phenol, 7 ketones, nitrogenous compound terpenes. The most abundant constituents short-chain acids (26.32%) while lowest (0.82%). principal component analysis (PCA) scores plot UPLC/MS dataset showed similarity bee's aroma profile. With regards antimicrobial assay, venom exerted strongest activity among examined S. K. A. MRSA but failing exert an effect case aforementioned bacteria.